A porcine spectral assay library to quantify brain proteome by DIA-MS
- Jakub Cervenka
- Rita Sucha
- Jirina Tyleckova
- Ievgeniia Poliakh
- Jaromir Novak
- Eva Kotrcova
- Karel Harant
- Pavel Rehulka
- Petr Vodicka
2026-08-24
Neurological disorders are the leading cause of health loss worldwide. The growing number of patients suffering from such conditions calls for improved strategies for their prevention, diagnosis, and therapy. To better understand human pathologies, relevant models and methodologies must be made available. In this study, we focused on a biomedical model capable of recapitulating the complexity of human pathology, the pig ( Sus scrofa ). Brain tissue and cerebrospinal fluid samples from a transgenic minipig model of Huntington’s disease were subjected to multiple extraction and fractionation steps. A proteomic mass spectrometry (MS) methodology then allowed the generation of a porcine spectral library for 8,321 proteins. Using data-independent acquisition (DIA), we demonstrated that our porcine spectral library substantially enhanced the quantitative potential of this untargeted MS approach, generating reproducible proteome-wide data. The porcine library also provides a comprehensive resource for the development of targeted MS assays, enabling the quantification of selected proteins with a key role not only in neuroscience.