A Proteomic Resource of Human iPSC-derived Neurons Exposed to Neurotropic Viruses
- Ziyi Li
- Negin P. Martin
- Jacob Epstein
- Shih-Heng Chen
- Ying Hao
- Daniel M. Ramos
- Erika Lara
- Kate M. Andersh
- Paige Jarreau
- Cory Weller
- Marianita Santiana
- Benjamin Jin
- Caroline B. Pantazis
- Luigi Ferrucci
- Mike A. Nalls
- Mark R. Cookson
- Andrew B. Singleton
- Yue Andy Qi
- Jerrel L. Yakel
2026-08-19
Viral infections are common across the human lifespan, yet their molecular effects on human neurons remain poorly characterized. We present a mass spectrometry (MS)-based proteomic dataset profiling human induced pluripotent stem cell (iPSC)-derived neurons following exposure to five neurotropic viruses: Herpes simplex virus 1 (HSV-1), Human coronavirus 229E (HCoV-229E), Epstein-Barr virus (EBV), Varicella-Zoster virus (VZV), and Influenza A virus (H1N1). Neurons differentiated from the KOLF2.1 J iPSC line were infected at multiple viral doses and sampled at three post-infection time points. Protein abundance was quantified using data-independent acquisition mass spectrometry and a customized human-viral proteome library, enabling simultaneous detection of host and viral proteins. The dataset includes approximately 7,500 human proteins and virus-specific peptides, providing a systematically controlled resource to compare virus- and time-dependent proteomic responses. All raw MS files and processed data are publicly deposited in the PRIDE repository, and an interactive web application supports data exploration and reuse. This dataset is designed to support cross-virus comparisons, hypothesis generation, and integration with transcriptomics and population-scale datasets.