A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling
- Miroslav Micka
- Jitender Kumar
- Petra Paclíková
- Zuzana Hayek
- Katerina Hanakova
- Cherine Bechara
- Hana Plešingerová
- Ondrej Šedo
- Sara Bologna
- Elise Del Nero
- Kristína Gömöryová
- Vojtěch Bystrý
- Tomáš Gybeľ
- Tereza Číhalová
- Marek Kravec
- David Potesil
- Zbynek Zdrahal
- Konstantinos Tripsianes
- Vítězslav Bryja
2026-05-13
It is well known that Dishevelled (DVL) phosphorylation by casein kinase 1 (CK1) relays Wnt signals from Frizzled (FZD) receptors to downstream effectors, yet any mechanistic aspects of DVL function related to phosphorylation remain unresolved. Here, we uncovered a Wnt-induced DVL phospho-switch that is mutually exclusive with FZD association. CK1 multiphosphorylation changes dramatically the bulk electrostatics to promote DVL intramolecular interaction between the DEP domain and the adjacent disordered region. A panel of DVL3 mutants demonstrated a switch-like behavior at the molecular level when a charge threshold was reached. Charge accumulation proximal to DEP proved to be a key functional event required, but not sufficient, for Wnt/β-catenin signaling. Proximity interactomics revealed FZD receptors as the prominent effectors of the DVL phospho-switch function consistent with the molecular competition at the DEP interface. By integrating findings at different levels, we propose a universal mechanism, in which Wnt-induced DVL conformational phospho-switch attenuates coresidence with FZDs as a means for downstream signaling events.