Allospecific splenic Tr1 cells drive effector T cell exhaustion through up-regulated Areg-EGFR signaling to promote transplant tolerance
- Amar Singh
- Adam Herman
- Devanjan Dey
- Samuel Menge
- Shilpi Singh
- Anna Tran
- Anders Matson
- Ahmed Mourad
- Parthasarathy Rangarajan
- Ravi Masuria
- Naoya Sato
- Sarah Vadnais
- Joseph Sushil Rao
- Gatikrushna Singh
- Melanie L. Graham
- Sabarinathan Ramachandran
- Bernhard J. Hering
2026-01-07
Inducing stable tolerance to transplants remains a challenge in immunology. Previously, we induced tolerance to allogeneic islets in nonhuman primates by preemptive alloantigen delivery to antigen-presenting cells in situ. Here, mass cytometry phenotyping with incorporated donor-derived MHC-I peptide–loaded MHC-II tetramers revealed accumulation of allospecific CD4 + T cell clusters in the spleen of tolerant recipients. Areg + Tr1 regulatory and terminally exhausted EGFR hi T (Tex) cells represented the predominant allospecific subsets. Trajectory analysis showed that antigen-experienced effector memory T cells differentiated into suppressive Areg + Tr1 and EGFR + TOX + Nur77 + TCF-1 − Tex subsets. Cell-cell communication mapping showed that exhausted and effector memory T cells engaged with allospecific Tr1 cells via the Areg-EGFR axis. Gene silencing studies confirmed that Tr1 cells use Areg-EGFR signaling to drive the metabolic suppression and epigenetic reprogramming of CD4 + T cells through a Nur77-dependent pathway. These findings point to the splenic Areg + Tr1 cell-EGFR + Teff cell axis as a critical immunoregulatory pathway in peripheral transplant tolerance.