PNAS

Direct dating of 3.5 Ga biogenic carbon in a microbial mat remnant, Singhbhum Craton, India

2026-08-17

Zircons interpreted to be of syn-volcanic origin recovered from the lower greenschist “black and white banded” Bhitardari chert, Eastern Iron Ore greenstone belt, Singhbhum craton, India, yield a 207 Pb/ 206 Pb zircon age of 3497 ± 5 Ma ( n = 4; MSWD = 1.2). This chert contains abundant carbonaceous matter (CM) that occurs in two textural modes: 1) ultrafine laminae distributed within the matrix and 2) graphite flakes in late quartz veins. Bulk δ 13 C CM of both modes in the Bhitardari chert yield a value of −30.9‰, which we interpret as reflecting a biogenic origin. This represents the documentation of a directly dated, CM-bearing, Paleoarchean rock with δ 13 C CM diagnostic of biologic activity. The observed isotopic compositions are consistent with the rise of relatively sophisticated metabolic mechanisms (e.g., Calvin cycle) by 3.5 Ga.

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DOI https://doi.org/10.1073/pnas.2617912123