Dynamic structures of a membrane transporter in native cellular membranes
- Huayong Xie
- Yuefang Gan
- Weijing Zhao
- Mojie Duan
- Yang Shen
- Huan Tan
- Yan Zhang
- Qiong Tong
- Yongxiang Zhao
- Jun Yang
2025-11-12
Substrate transport through membrane transporters is a dynamic process that involves transitions between different functional conformations, which can be disrupted by non-native membrane mimetics. Capturing these conformations and their transitions within native cellular membranes presents a notable challenge. Herein, we used in situ solid-state nuclear magnetic resonance (NMR) to resolve the 1.5-Å outward-open and 2.5-Å occluded structures of Bj SemiSWEET within its native cellular membranes. Our findings reveal that these two conformations exchange within transmembrane helix TM1 and Loop L2-3 on a millisecond to second timescale, with the exchange rate corresponding to the sucrose transport rate. Molecular dynamics simulations further confirmed that these conformations represent functional states during sucrose transport. In contrast, we observed different conformational dynamics of Bj SemiSWEET in DMPC/DMPG synthetic bilayers compared to cellular membranes. This study highlights the potential of in situ solid-state NMR to provide previously unknown dynamic structural insights into cellular molecular processes, representing a substantial advancement in dynamic cellular structural biology.