Fast growth and high-titer bioproduction from renewable formate via metal-dependent formate dehydrogenase in Escherichia coli
- Aidan E. Cowan
- Mason Hillers
- Vittorio Rainaldi
- Florent Collas
- Hemant Choudhary
- Basem S. Zakaria
- Gregory G. Bieberach
- David N. Carruthers
- Maxwell Grabovac
- Jennifer W. Gin
- Bridgie Cawthon
- Yan Chen
- Emine Akyuz Turumtay
- Edward E. K. Baidoo
- Christopher J. Petzold
- Adam M. Feist
- Sara Tejedor-Sanz
- Frank Kensy
- Blake A. Simmons
- Jay D. Keasling
- Nico J. Claassens
2025-07-01
Microbial bioproduction using one-carbon (C1) feedstocks has the potential to decarbonize the manufacturing of materials, fuels, and chemicals. Formate is a promising C1 feedstock, and the realization of industrial, formatotrophic platform organisms is a key goal for C1-based bioproduction. So far, a major limitation for synthetic formatotrophy has been slow energy supply due to slow formate dehydrogenase activity. Here, we implement a fast, metal-dependent formate dehydrogenase complex in a synthetic formatotrophic Escherichia coli utilizing the reductive glycine pathway. After a short-term evolution, we demonstrate formatotrophic growth of E. coli with a doubling time of less than 4.5 h, comparable to the fastest natural formatotrophs. To further explore the potential of a formate-based bioeconomy, this strain is engineered to produce mevalonate, as well as the terpenoid and aviation fuel precursor isoprenol, using formate we generate directly from the electrochemical reduction of CO 2 . This work demonstrates an improvement in bioproduct titer from formate, achieving the production of 3.8 g/L of mevalonate. Additionally, the abundant and recalcitrant polymer lignin is chemically decomposed into a formate-rich mixture of small organic acids and subsequently bioconverted into mevalonate. Overall, the described fast-growing, formatotrophic bioproduction strain demonstrates that a sustainable formate bioeconomy is within reach.