Functional chimeric mRNAs encode proteins in mammalian immunity
- Olivia Venezia
- Harry Kane
- Gang Du
- Hannah D. Coughlan
- Timothy M. Johanson
- Hongshen Wang
- Pathricia Tilstam
- Samuel W. Kazer
- Georgia Gunner
- Daisy A. Hoagland
- Megha G. Basavappa
- Kanchana Ravichandran
- Eren Ada
- Ademi Zhakyp
- Sandhya Kumar
- Coco Duizer
- Owen Searle
- Chris Geo Provido
- Leonel Joannas
- Shilong Yang
- Liam B. Healy
- Qiankun Wang
- Joia Capocchi
- Bahawar Sharif Dhillon
- Sarah Slavoff
- Liang Shan
- Jorge Henao-Mejia
- Ruth A. Franklin
- Isaac M. Chiu
- Jose Ordovas-Montanes
- Kate L. Jeffrey
- Judy Lieberman
- Rhys S. Allan
- Jonathan C. Kagan
- Hao Wu
- Ruaidhrí Jackson
2026-09-02
Individual mammalian mRNAs and proteins are typically believed to originate from single genomic loci, with isoform diversity arising through cis -splicing of pre-mRNA. Whether mRNA from distant genes can undergo trans -splicing to generate functionally relevant chimeric transcripts has remained unclear. Here we develop a pipeline combining long-read direct RNA sequencing with non-targeted and targeted validation to identify chimeric transcripts in macrophages. Chromatin conformation capture studies reveal that inflammation induces interchromosomal DNA interactions, positioning parent genes proximally to facilitate the formation of chimeric mRNA. Notably, we identify a protein-coding chimeric mRNA representing a fusion between the pore-forming protein gasdermin D (GSDMD) 1,2 and a C-terminal domain translated out of frame from Tmem106a ( Gsdmd - Tmem106a ) in mice. We show that inflammasome priming upregulates Gsdmd - Tmem106a , with the protein localizing to the plasma membrane. After activation of the inflammasome, GSDMD–TMEM106A directly interacts with canonical GSDMD N termini to accelerate and enhance pore formation and IL-1β release. Finally, we show that GSDMD–TMEM106A balances host defence and immunopathology in vivo: its loss protects against lethal sepsis but compromises antibacterial defence, whereas overexpression enhances host protection while increasing sepsis lethality. We establish that protein-coding chimeric mRNAs formed by regulated transcript fusion events are operative during inflammation and immunity.