Global dataset of soil eukaryotic communities created with a uniform protocol and long read sequencing
- Vladimir Mikryukov
- Olesya Dulya
- Kessy Abarenkov
- Sten Anslan
- Niloufar Hagh-Doust
- Victoria Prins
- Kristel Panksep
- Sergei Põlme
- Khalid S. Ibrahim
- Mo Bahram
- Kalev Adamson
- Ahto Agan
- Talaat Ahmed
- Juha M. Alatalo
- Felipe E. Albornoz
- Abdullah MS Al-Hatmi
- Saad Alkahtani
- Julieta Alvarez-Manjarrez
- Jelena Ankuda
- Alexandre Antonelli
- Manikandan Ariyan
- Kęstutis Armolaitis
- Farzad Aslani
- Isabel C. Barrio
- Marijn Bauters
- Elisabeth Machteld Biersma
- Krišs Bitenieks
- Gregory Bonito
- Francis Q. Brearley
- Kari Anne Bråthen
- Franz Buegger
- Klaus Butterbach-Bahl
- Miklós Bálint
- Erin K. Cameron
- Fabiana Canini
- Rebeca Casique-Valdés
- Adriana Corrales
- Evgeny A. Davydov
- Eske De Crop
- André De Kesel
- Joseph Fovo Djeugap
- Rein Drenkhan
- Camila Duarte Ritter
- Sergey V. Dudov
- Mikk Espenberg
- Ongua Fanuel
- Vladimir E. Fedosov
- Luke Florence
- Brendan R. Furneaux
- Ariadne N. M. Furtado
2026-05-05
Soil eukaryotes, including fungi, protists, plants, and animals, are central to biosphere functioning and resilience. The Global Standardised Soil Eukaryome Dataset (GloSED) is the first dataset encompassing the entire spectrum of soil eukaryotes, covering 4,063 sampling sites in 121 countries on all continents, revealing nearly one million operational taxonomic units. All samples were collected and analysed using a standardised protocol minimizing technical biases. Long-read sequencing of full-length ITS and 18S-V9 regions provide broad taxonomic coverage and high-resolution identification supported by specialist curation of “dark taxa”. A rigorous bioinformatic processing ensures against homopolymer errors, PCR-mediated chimeras, and index switching providing high data quality. The dataset is supported by raw sequences and an open-source containerised workflow for reproducible analyses. The samples are accompanied by land-cover description and directly measured soil pH, δ 13 C, δ 15 N, as well as P, K, Ca, Mg, and total C and N contents. GloSED is the first database that enables ecological and biogeographic studies of entire soil eukaryotic communities from local to global scales.