HSPCs and T reg cells cooperate to preserve extramedullary hematopoiesis under chronic inflammation
- Maria Kuzmina
- Srdjan Grusanovic
- Jiri Brezina
- Flavian Thelen
- Karolina Vanickova
- Mirko Milosevic
- Irina Ribeiro Bas
- Nataliia Pavliuchenko
- Sarka Ruzickova
- Jakub Rohlena
- Dominik Filipp
- Katerina Rohlenova
- Cesar Nombela-Arrieta
- Tomas Brdicka
- Meritxell Alberich-Jorda
2026-05-06
Hematopoietic stem and progenitor cells (HSPCs) are localized within specialized niches of the bone marrow (BM). However, during hematological disorders or infections, the functionality of these cells in the BM is compromised, leading to extramedullary hematopoiesis (EMH). Chronic inflammation drives EMH, yet its impact on HSPCs outside the BM is poorly understood. Using a mouse model of chronic autoinflammatory disease, we demonstrated the presence of extramedullary HSPCs in blood, spleen, and inflamed tails and paws. Single-cell transcriptomics revealed a unique expression profile in extramedullary HSCs, with significant up-regulation of Cd53 , MHCII-associated, and immunosuppressive genes. We further demonstrated that extramedullary CD53 + HSPCs act as antigen-presenting cells, promoting the development of regulatory T cells (T reg cells) to control chronic inflammation at extramedullary sites. Conversely, T reg cells exert a protective role on extramedullary HSPCs. Together, our findings revealed a mutually supportive relationship between a unique subset of HSPCs and T cells in inflamed tissues during chronic inflammation.