Indolent primary cutaneous B-cell lymphomas resemble persistent antigen reactions without signs of dedifferentiation
- Johannes Griss
- Sabina Gansberger
- Inigo Oyarzun
- Martin Simon
- Mathias C. Drach
- Vy Nguyen
- Lisa E. Shaw
- Ulrike Mann
- Stefanie Porkert
- Matthias Farlik
- Wolfgang Weninger
- Werner Dolak
- Bertram Aschenbrenner
- Beate M. Lichtenberger
- Shawn Ziegler-Santos
- Christine Wagner
- Ingrid Simonitsch-Klupp
- Stephan N. Wagner
- Constanze Jonak
- Patrick M. Brunner
2026-02-04
Primary cutaneous B-cell lymphoma encompass clinically heterogeneous entities. While primary cutaneous diffuse large B-cell lymphoma, leg type (pcDLBCL-LT) is aggressive, primary cutaneous follicle centre lymphoma (pcFCL) and primary cutaneous marginal zone lymphoma (pcMZL) typically follow an indolent course. To clarify their pathophysiological basis, we perform single-cell RNA sequencing on pcFCL, pcMZL, and pcDLBCL-LT, alongside reactive B-cell rich lymphoid proliferations (rB-LP), gastric mucosa-associated lymphoid tissue (MALT) lymphoma, and systemic counterparts. Here we show that the indolent pcMZL, pcFCL, and rB-LP exhibit a persistent germinal centre reaction, not observed in pcDLBCL-LT or gastric MALT lymphoma. Further, pcMZL top expanded clones develop within lesions from naïve and not post-germinal centre B cells as currently presumed. Our data thus indicate that pcMZL and pcFCL, similar to rB-LP may be driven by (a yet unknown) antigen. While our data indicates that pcFCL exhibits some features of true lymphomas, it clearly supports the classification of pcMZL as a lymphoproliferative disease.