Kitlo hematopoietic stem cells exhibit distinct lymphoid-primed chromatin landscapes that enhance thymic reconstitution
- Harold K. Elias
- Sneha Mitra
- Marina B. da Silva
- Adhithi Rajagopalan
- Brianna Gipson
- Nicole Lee
- Anastasia I. Kousa
- Mohamed A. E. Ali
- Simon Grassmann
- Rhoshini Raghuraman
- Xiaoqun C. Zhang
- Susan DeWolf
- Melody Smith
- Hana Andrlova
- Kimon V. Argyropoulos
- Roshan Sharma
- Teng Fei
- Joseph C. Sun
- Cynthia E. Dunbar
- Christopher Y. Park
- Christina S. Leslie
- Avinash Bhandoola
- Michael G. Kharas
- Marcel R. M. van den Brink
2025-07-04
Hematopoietic stem cells (HSC) with multilineage potential are critical for T cell reconstitution after allogeneic hematopoietic cell transplantation (allo-HCT). The Kit lo HSC subset is enriched for multipotential precursors, but their T cell potential remains poorly characterized. Using a preclinical allo-HCT mouse model, we demonstrate that Kit lo HSCs provide superior thymic recovery and T cell reconstitution, resulting in improved immune responses to post-transplant infection. Kit lo HSCs with augmented bone marrow (BM) lymphopoiesis mitigate age-associated thymic alterations and enhance T cell recovery in middle-aged mice. Mechanistically, chromatin profiling reveals Kit lo HSCs exhibiting higher activity of lymphoid-specifying transcription factors, such as, ZBTB1. Zbtb1 deletion diminishes HSC engraftment and T cell potential; by contrast, reinstating Zbtb1 in megakaryocytic-biased Kit hi HSCs rescues hematopoietic engraftment and T cell potential in vitro and in vivo. Furthermore, age-associated decline in Kit lo HSCs is associated with diminished T lymphopoietic potential in aged BM precursors; meanwhile, Kit lo HSCs in aged mice maintain enhanced lymphoid potential, but their per-cell capacity is diminished. Lastly, we observe an analogous human BM KIT lo HSC subset with enhanced lymphoid potential. Our results thus uncover an age-related epigenetic regulation of lymphoid-competent Kit lo HSCs for T cell reconstitution.