Multiorgan transcriptomics in mice identifies immunoglobulin heavy constant mu ( Ighm ) as a tissue-level aging biomarker
- Fan-Qian Yin
- Xia-Yan Wang
- Yong-Xuan Li
- Kai-Jing Li
- Si-Yu Ma
- Meng-Jiao Lv
- Zhen-Hua Liu
- Wei-Xia Zhong
- Yan Liu
- Chuan-Fang Tang
- Hong-Shi Liu
- Yuze Li
- Fu-Hui Xiao
- Qing-Peng Kong
2025-07-11
Identifying aging-associated biomarkers applicable for multiple tissues is challenging but crucial for assessing tissue aging. Here, we obtained and analyzed 456 transcriptomes on 17 organs from 30 C57BL/6 J mice with different ages, revealing the consistently upregulated mRNAs of Ighm , C4b , and Ccl8 in most aged organs. This finding received support from independent transcriptomic and proteomic datasets and was further validated through western blot, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence, arguing for both Ighm mRNA and protein as tissue-level aging biomarkers, at least in mice. Its sensitivity to antiaging interventions further emphasizes the significance of Ighm in assessing tissue aging in mice.