Pentavalent and tetravalent uranium formation via glycerol-stimulated bacteria in mine water
- Antonio M. Newman-Portela
- Kristina O. Kvashnina
- Elena F. Bazarkina
- André Rossberg
- Frank Bok
- Sean Ting-Shyang Wei
- Andrea Kassahun
- Thorsten Stumpf
- Johannes Raff
- Mohamed L. Merroun
- Evelyn Krawczyk-Bärsch
2026-05-04
Uranium contamination is a major global concern, as its chemical and radiological effects threaten ecosystems and human health, particularly in mining-impacted regions. Pentavalent uranium is a key but often overlooked intermediate in U biogeochemistry, challenging the conventional view of a direct U(VI) to U(IV) transition and precipitation in natural waters. Here we demonstrate the formation and stability of U(V) under environmentally relevant mine-water conditions using advanced spectroscopic and microscopic analyses. Our data reveal concurrent U(VI) reduction to U(IV), as biogenic uraninite nanoparticles, and to U(V) as FeU (V) O₄ nanoparticles and U(V)-carbonate complexes. U(V) persists for at least 130 days under anoxic conditions and four weeks after exposure to oxygen. Microbial community analysis reveals enrichment of fermentative and sulphate-reducing taxa, consistent with redox conditions favouring U reduction. By demonstrating the stability of immobilised U(V) alongside U(IV), this work advances the understanding of uranium biogeochemistry and offers new insights for sustainable remediation strategies.