Porin A and α/β-hydrolase are necessary and sufficient for hemolysis induced by Bartonella bacilliformis
- Alexander A. Dichter
- Florian Winklmeier
- Diana Munteh
- Wibke Ballhorn
- Sabrina A. Becker
- Beate Averhoff
- Halvard Bonig
- Adrian Goldman
- Meritxell García-Quintanilla
- Luis Solis Cayo
- Pablo Tsukayama
- Volkhard A. J. Kempf
2025-11-28
Carrion’s disease is endemic to the South American Andes and is characterized by fatal hemolytic anemia. This neglected tropical disease is caused by Bartonella bacilliformis , a fastidious and slow-growing pathogen difficult in genetic manipulation. In this study, we determine that porin A and α/β-hydrolase are both necessary and sufficient for hemolysis induced by B. bacilliformis . These genes are identified through a screen of a Tn5 transposon mutant library. Using markerless deletion mutagenesis, porin A and α/β-hydrolase deletion mutants are generated and functionally analyzed by hemolysis assays. In silico analyses predict conserved biological functions and three-dimensional structures of the identified proteins, with the α/β-hydrolase showing structural similarity to known lipases. Site-directed mutagenesis of the α/β-hydrolase active site demonstrates that the catalytic triad (Ser205, Asp267, His310) is essential for its hemolytic function. Screening of a phospholipase inhibitor library comprising 27 bioactive compounds identifies compound 48/80 as a potent inhibitor of hemolysis, with activity in the micromolar range. Unraveling the molecular mechanisms underlying Carrion’s disease may facilitate the future development of anti-virulence therapies, a promising strategy particularly in the context of increasing antibiotic resistance of B. bacilliformis .