Rapid model-guided design of organ-scale synthetic vasculature for biomanufacturing
- Zachary A. Sexton
- Dominic Rütsche
- Jessica E. Herrmann
- Andrew R. Hudson
- Soham Sinha
- Jianyi Du
- Daniel J. Shiwarski
- Anastasiia Masaltseva
- Fredrik Samdal Solberg
- Jonathan Pham
- Jason M. Szafron
- Sean M. Wu
- Adam W. Feinberg
- Mark A. Skylar-Scott
- Alison L. Marsden
2025-06-18
Our ability to produce human-scale biomanufactured organs is limited by inadequate vascularization and perfusion. For arbitrarily complex geometries, designing and printing vasculature capable of adequate perfusion poses a major hurdle. We introduce a model-driven design platform that demonstrates rapid synthetic vascular model generation alongside multifidelity computational fluid dynamics simulations and three-dimensional bioprinting. Key algorithmic advances accelerate vascular generation 230-fold and enable application to arbitrarily complex shapes. We demonstrate that organ-scale vascular network models can be generated and used to computationally vascularize >200 engineered and anatomic models. Synthetic vascular perfusion improves cell viability in fabricated living-tissue constructs. This platform enables the rapid, scalable vascular model generation and fluid physics analysis for biomanufactured tissues that are necessary for future scale-up and production.