Small-molecule allosteric activator of ubiquitin-specific protease 7 (USP7)
- Isabella Jaen Maisonet
- Mona Sharafi
- Emilie J. Korchak
- Andres Salazar-Chaparro
- Ariana S. Bratt
- Tvesha Parikh
- Anthony C. Varca
- Binita Shah
- Michael Darnowski
- Mia Chung
- Wei Pin Teh
- Jianwei Che
- Irina Bezsonova
- Sara J. Buhrlage
2025-10-14
Ubiquitin-specific protease 7 (USP7) is a deubiquitylase essential for cell homeostasis, DNA repair, and regulation of both tumor suppressors and oncogenes. Recently, haploinsufficiency of USP7 has been associated with Hao–Fountain syndrome (HAFOUS), a rare neurodevelopmental disorder. Although a range of USP7 inhibitors have been developed over the last decade, in the context of HAFOUS, USP7 activators may represent a more relevant approach. To address this challenge, we report the identification and characterization of a small-molecule activator of USP7 called MS-8. Structural and functional studies show that MS-8 allosterically activates USP7, mimicking the endogenous autoactivation mechanism of the enzyme. We observed that MS-8 engages and activates mutant USP7 in a cellular context, impacting downstream proteins. Taken together, our study provides validation of the USP7 activator that paves the way toward activation-driven USP7 pharmacology.