Sorting of lysosomal enzyme and autophagy are regulated by the GGA1-induced TGN lipid scrambling
- Kohta Takahashi
- Nario Tomishige
- Mitsuhiro Abe
- Gašper Šolinc
- James Rae
- Toshiyuki Yamaji
- Frédéric Przybilla
- Ludovic Richert
- Nicolas Humbert
- Takefumi Uemura
- Thomas Wollert
- Satoshi Waguri
- Kentaro Hanada
- Yasushi Sako
- Gregor Anderluh
- Robert G. Parton
- Yves Mély
- Catherine Tomasetto
- Fabien Alpy
- Toshihide Kobayashi
2026-08-28
The physiological role of lipid asymmetry in intracellular membranes remains poorly understood. Here, we show that sphingomyelin (SM), typically confined to the lumen of the trans-Golgi network (TGN), is exposed on its cytoplasmic surface by the action of the Golgi-associated protein, Golgi-associated gamma-adaptin ear–containing adenosine 5′-diphosphate–ribosylation factor–binding protein 1 (GGA1). This exposure is driven by the GGA1 GAT domain, which induces lipid scrambling in a manner dependent on membrane curvature and cholesterol. SM exposure coincides with the exit of mannose 6-phosphate receptors from the TGN, a process essential for lysosomal enzyme trafficking. Furthermore, SM is transferred to autophagic membranes, where it facilitates autophagosome-lysosome fusion. These findings reveal a previously unrecognized role for lipid remodeling in membrane trafficking and autophagy.