The confounding effects of skin colour in photoacoustic imaging
- Thomas R. Else
- Christine Loreno
- Alice Groves
- Benjamin T. Cox
- Evangelia Vetsiou
- Janek Gröhl
- Inés Modolell
- Sarah E. Bohndiek
- Amit Roshan
2026-06-17
Skin colour is known to confound optical devices, adversely impacting care for patients with darker skin. Photoacoustic imaging (PAI) combines optics and ultrasound for deep tissue imaging, creating a complex relationship between PAI-derived biomarkers and skin melanin concentration, yet no generalisable bias correction has been demonstrated. Drawing on a healthy volunteer cohort of 42 participants spanning Fitzpatrick types I–VI and vitiligo – the most diverse ever assembled in PAI – we characterise optical and acoustic mechanisms driving skin colour-dependent degradation in image quality and biomarker quantification. Wavelength-dependent melanin absorption causes spectral colouring, dominating at low melanin levels, while epidermal ultrasound backscattering dominates at high melanin levels, producing a non-linear relationship between unmixed sO₂ and skin tone. Leveraging this understanding, we propose a practical spectral colouring correction and adapt a plane-wave reconstruction algorithm to resolve backscattered ultrasound artefacts. Our findings underscore the need for advanced reconstruction methods to enable equitable clinical PAI.