Three cryo-EM structures of complement C3d-bound α M β 2 reveal an unexpected layer of dynamics for αI-containing integrin receptors
- Josefine Lorentzen
- Marlene Uglebjerg Fruergaard
- Szilvia Lukácsi
- Martin Høgholm Jørgensen
- Timo Lambertus Gerardus van Veghel
- Rasmus Kjeldsen Jensen
- Krzysztof Jakub Pietrzak-Lichwa
- Zsuzsa Bajtay
- Václav Hořejší
- Rasmus Kock Flygaard
- Daan Vorselen
- Simon Arnold Mortensen
- Gregers Rom Andersen
2026-05-12
Integrins are heterodimeric membrane proteins acting as mechanosensing receptors. Nine human α-subunits contain a ligand binding αI domain, but how ligands activate αI integrins are not understood. We present cryo-EM structures of the αI integrin α M β 2 in complex with the C3d ligand. The ligand-bound αI domain appears to have two major opposite orientations relative to the β 2 subunit. Ligand binding induces an ordered conformation of the α M internal ligand region that is tightly packed between the α M β-propeller and the β 2 βI-domain. Recognition of the internal ligand induces an open βI conformation practically identical to that of ligand-bound αI-less integrins confirming that ligand binding and signaling are coupled by a universal mechanism across all integrins. Integration of our findings with prior data allows us to propose a model for C3dg/iC3b-bound α M β 2 in the phagocytotic cup and outline mechanistic models for external ligand-induced activation of α M β 2 .