Two-step voltage-sensor activation of the human KV7.4 channel and effect of a deafness-associated mutation
- Mario Nappi
- Damon J. A. Frampton
- Ali S. Kusay
- Kaiqian Wang
- S. Suheda Yasarbas
- Serena Pozzi
- Francesco Miceli
- Sara I. Liin
- Maurizio Taglialatela
- Antonios Pantazis
2026-02-05
KCNQ4 -encoded K V 7.4 voltage-gated potassium channels are expressed in hair-cells of the inner ear. Loss-of-function variants in KCNQ4 cause non-syndromic progressive hearing loss (DFNA2). K V 7.4 pore opening requires voltage-dependent conformational changes (activation) of the voltage-sensor domains (VSDs); however, how fast charge displacement during VSD activation is coupled to slow channel opening is currently unclear. Here, we optically tracked K V 7.4 VSD activation with voltage-clamp fluorometry, leveraging two fluorophores and pulsed excitation, and found that VSD activation comprises several voltage-dependent transitions, some with kinetics and voltage-dependence matching those of channel opening and closing. The DFNA2-causing R216H mutation impairs VSD movement and channel opening by destabilizing the active VSD configuration, a result confirmed by molecular dynamics simulations. We propose that the K V 7.4 VSD activates in two steps: a fast movement representing a first transition to an intermediate activation state, followed by slower component(s) that fully activate the VSD and drive channel opening.