Unified down-stream analysis of crosslinking mass spectrometry results with pyXLMS
- Micha J. Birklbauer
- Louise M. Buur
- Sabrina Kaser
- Fränze Müller
- Manuel Matzinger
- Karl Mechtler
- Stephan Winkler
- Viktoria Dorfer
2026-09-04
Crosslinking mass spectrometry has become the method of choice for the identification of protein-protein interactions and for gaining insight into the structures of proteins in vivo. However, connecting crosslink search engine results with down-stream analysis tools, and therefore gaining biological insight from crosslink identifications, has remained a manual and cumbersome step in the analysis that often requires expert bioinformatics knowledge. Here we introduce pyXLMS, a python package and public web application which aims to simplify and streamline this intermediate step, enabling researchers even without bioinformatics knowledge to conduct in-depth crosslink analyses. In its current state pyXLMS supports input from more than seven different crosslink search engines, as well as the mzIdentML format of the HUPO Proteomics Standards Initiative. Data processing and quality control is facilitated by functionality that is directly available within pyXLMS such as aggregation, validation, annotation, filtering, and visualization. In addition, the data can easily be exported to more than ten supported down-stream analysis tools and formats. We demonstrate the applicability and benefits of pyXLMS by re-analyzing a publicly available crosslink dataset with a variety of different search engines and show how the same data analysis workflow can be applied using pyXLMS.